# Hua's Cabin

### Differential Expression Analysis

1. 如果数据存在配对情况，则可设置pair=T。

2. scale参数是针对counts数据设置的。

3. future.apply是对并行计算设置的。

4. eBayes的结果中logFC有时候让人很困惑，需要注意该结果是否的正确性，因此设置datCoe判断富集方向，也可以通过内置的pl图发现富集方向。

### Welch’s t-test

The independent samples t-test comes in two different forms:
the standard Student’s t-test, which assumes that the variance of the two groups are equal. the Welch’s t-test, which is less restrictive compared to the original Student’s test. This is the test where you do not assume that the variance is the same in the two groups, which results in the fractional degrees of freedom.

### Reference

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